Coding

Part:BBa_K812054

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-20)


RFP E1010 with GoldenBrick sequences

RFP E1010 with GoldenBrick extension

This part is the GoldenBricked version of E1010 RFP. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.

To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
    Illegal AgeI site found at 572
    Illegal AgeI site found at 684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 706


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